MicroRotofor™ Cell Lysis Kits
MicroRotofor lysis kits provide cell lysis and protein extraction protocols that are tailored to the specific needs of different sample sources. Convenient and easy to use, all four kits are based on the same chaotropic protein solubilization buffer (PSB), which contains nondetergent sulfobetaine 201 (NDSB 201) along with urea, thiourea, and CHAPS for particularly effective solubilization (Vuillard et al. 1995). The kits generate total protein samples that are ready for fractionation with the MicroRotofor liquid-phase IEF cell, but they may also be applied to other downstream applications, such as SDS-PAGE and 2-D gel electrophoresis.