Genetically Modified Organisms

Genetically modified organisms

Innovative solutions for the detection of GMO in food 

The world wide production of genetically modified food (e.g. soy and corn) has steadily increased since the beginning of the 90’s.

Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labelled. As stated by the Council of Ministers of the European Union (EU), regulation for the labelling of Novel Food: Decision No. 1139/98/EC. Furthermore, since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0.9 % in EU for food and feed.

Qualitative GMO detection

According to national or regional regulations, zero tolerance applies to non-approved GMO events. As such, only qualitative tests are of interest for these products at the moment. The detection limit lies at 0.01 %, depending on the matrix and the application.

Regulation EC 619/2011 exists as a special rule for animal feeds. Provided that certain conditions are met (expired EU approval, approval in the country of origin, availability of tests and reference material, etc.), a technical threshold value of 0.1 % applies for non-approved GMO events. This is particularly significant for not always contamination-free imports of GMOs and GMO-free maize and soya.

Quantitative GMO detection

For respectively approved GMO events, there is the quantitative question of whether the product lies above the defined threshold values or mandatory labelling values.

In Europe, a value of 0.9 % applies to the GMO event in the respective plant matrix. Below that value, foodstuffs with technically unavoidable and coincidental contaminations of the GMO events do not need to be declared (EC 2829/2003 und EC2830/EC). The quantification occurs via the respective quantification of a GMO specific and plant matrix specific gene based on a respectively different calibration curve and the conversion to GMO plant /matrix DNA number of copies. This dimensionless unit multiplied by 100 yields the percentage value. The calibration curve is obtained via the dilution of a DNA standard and, on the basis of a defined GMO material, can also be specified precisely with an experimental correction factor.

GMO screening

A screening of plant extrinsic gene sequences that were infiltrated into the plants during GMO development provides efficient evidence of the presence of any possible GMOs.

The typically used gene sequences are:

  • 35S
  • NOS
  • FMV
  • BAR
  • CTP2:CTP4 EPSPS

These and further parameters can either be combined in a modular way or used individually. The efficiency of the DNA preparation can be verified with a plant-specific PLANT Plus Kit (S2049). The PLANT Kit (S2056) is sufficient for the 4plex Kit with internal amplification control.

SureFood® is a modular system which is based on the 3 main components , amplification and detection. Each component consists of different modules. All modules are compatible. Thus, SureFood® is a highly flexible system which can be easily configured for different user specific applications. SureFood® is available as product and service as well.

SureFood® GMO product overview

You can find a comprehensive overview of our SureFood® GMO test kits in our free brochure.

 

ORDER ENQUIRES:

ROI orders / enquiries please contact

+353 1 2907000

+353 1 2907111

NI orders / enquiries please contact

+44 2890 735 588

+44 2890 735 599


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